Diisocyanate Specific Monoclonal Antibodies for Occupational and Environmental Monitoring of Polyurethane Production Exposure-related Asthma and Allergy and Clinical Diagnosis

Diisocyanate Specific Monoclonal Antibodies for Occupational and Environmental Monitoring of Polyurethane Production Exposure-related Asthma and Allergy and Clinical Diagnosis

CDC researchers have developed monoclonal antibodies useful as diagnostics for diisocyanate (dNCO) exposure and for toxicity characterization of specific dNCOs. Currently, dNCOs are used in the production of all polyurethane products and are the most commonly reported cause of occupational-induced asthma and also linked to allergic contact dermatitis. Presumptive diagnosis of dNCO asthma is presently dependent on criteria such as work history, report of work-related asthma-like symptoms and nonspecific airway reactivity to methacholine challenge.

This invention is a cost-effective, objective alternative for clinical assessment of occupational/environmental dNCO exposure in patient samples. These antibodies may also provide for passive-immunization and prevention of allergic contact dermatitis and/or asthma that can result from extended dermal exposure to dNCO contaminated surfaces and vapors. Further, the present technology allows for high-throughput testing of workplace dNCO air, fabric and working-surface contamination.

Applications

  • Occupational/environmental safety biomonitoring of polyurethane-worker/user exposure to diisocyanates(dNCOs)
  • Clinical diagnostic use
  • dNCO-induced allergy/asthma prevention by passive immunization

Advantage

  • Ready for use in high-throughput immuno-histochemistry biomarker detection assays and kits
  • Two sandwich ELISAs have been developed and validated using human samples
  • Monitoring is currently performed by elaborate analytical chemical assays; this technology is more rapid and cost effective for dNCO exposure/contamination assessment

For more details and contact information, visit http://www.ott.nih.gov/technology/e-189-2013

Benefits: 
Ready for use in high-throughput immuno-histochemistry biomarker detection assays and kits. -Two sandwich ELISAs have been developed and validated using human samples. -Monitoring is currently performed by elaborate analytical chemical assays; this technology is more rapid and cost effective for dNCO exposure/contamination assessment.
applications: 
Inventors: 
Paul Siegel
Internal Laboratory Ref #: 
E-189-2013/0
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