A Novel Fusion Protein for Inhibiting HIV Budding

Ubiquitin plays a critical role in HIV-1 budding. Vectors containing deubiquitin enzymes (DUbs) were constructed to deliver DUbs to HIV-1 production sites in living cells. The DUbs vectors comprise DUb cDNAs and cDNA expressing either HIV-1 gag, or the ESCRT protein TSG101. Experimental data show that the fusion proteins expressed by the DUbs vectors retained their known protein-protein interactions and caused a significant and specific interruption of HIV-1 budding. The data suggest that the DUbs vectors could be used to inhibit HIV-1 infection or propagation in an individual. Thus, the DUbs vectors could potentially be used in high-risk individuals to prevent HIV-1 infection or as an adjunct therapy with known Anti-Retroviral Therapy (ART/HAART) in infected individuals.
Abstract: 
Ubiquitin plays a critical role in HIV-1 budding. Vectors containing deubiquitin enzymes (DUbs) were constructed to deliver DUbs to HIV-1 production sites in living cells. The DUbs vectors comprise DUb cDNAs and cDNA expressing either HIV-1 gag, or the ESCRT protein TSG101. Experimental data show that the fusion proteins expressed by the DUbs vectors retained their known protein-protein interactions and caused a significant and specific interruption of HIV-1 budding. The data suggest that the DUbs vectors could be used to inhibit HIV-1 infection or propagation in an individual. Thus, the DUbs vectors could potentially be used in high-risk individuals to prevent HIV-1 infection or as an adjunct therapy with known Anti-Retroviral Therapy (ART/HAART) in infected individuals.
Benefits: 
Use for both treatment and prevention -No development of resistance to HIV
applications: 
Inventors: 
Fadila Bouamr
Patent Number: 
PCT/US2015/042492
Internal Laboratory Ref #: 
E-223-2014/0
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