The Naval Research Laboratory (NRL) has successfully developed a magnetic microbead support that enables the capture of genomic targets in a single step. The microbead is functionalized with a phosphorous branched dendrimer linker that covalently binds DNA capture probes, allowing hybridization of the target directly onto the bead. The targets can then be recovered through heat denaturation in small elution volumes for direct analysis.
The robust bond between capture probe and microbead permits the regeneration of the probe even after repeated cycles at high temperatures (up to 100°C) and salt conditions (3-5 M NaCl), providing a reusable universal platform for sequencecapture or background subtraction. Use of variable branched dendrimeric linker systems enables the performance to be tailored for specific targets. NRL is seeking patent licensees or CRADA partners.
For further information, contact the Technology Transfer Office at the Naval Research Laboratory, techtran@utopia.nrl.navy.mil.
