Invented by National Cancer Institute inventors Dimiter S. Dimitrov and Weizao Chen, and available for licensing and commercial development, is a highly diverse domain antibody (dAb) library providing antibodies that bind with high affinity to a variety of antigen targets. Antibody diversity is inherently limited by using only three CDRs of either light chain variable domain (LCDRs) or heavy chain variable domain (HCDRs).
This novel dAb library is designed using light chain variable domain 3 (LCDR3) and heavy chain variable domain (HCDR3), which are of primary importance for creating binding site diversity in the human immune system. The library contains 2.5 x 1010 dAbs. Human naturally occurring LCDR3s were grafted onto HCDR1 of m0.
These antibodies are of very small size (15-17 kDa), high stability and can be expressed at high levels as monomers. The library can be used for the selection of antibodies to any antigen, including cancer and viral antigens, and exhibit such properties as good penetration, stability, solubility, high levels of expression (at potentially low cost), and low level of immunogenicity or toxicity.
More info: Michael A. Shmilovich, Esq.; 301-435-5019; shmilovm@mail.nih.gov
